New NordVal International certificates
New Nordval Certificate 049 issued to BioRad for RAPID´Staph. RAPID’Staph is based on enumeration on an optimised Baird-Parker formula for enumeration of S. aureus in 24 h at 37° C. Some Staphylococcus coagulase positive strains can give colonies with a small or no halo after 24 hours of incubation. An additional incubation of 24 hours could be necessary to see the halo. RAPID´Staph is applicable for a broad range of foods. The validation is performed according to ISO 16140-2 and NordVal Validation Protocol 1. See the certificate here.
NordVal International Certificate 022 issued to BioRad for RAPID´L. mono has been renewed. A renewal study was carried out according to ISO 16140-2:2016 and NordVal Validation Protocol 1 to test for relative trueness and accuracy profile. The principle of the RAPID’L. mono medium is a chromogenic detection of the Listeria monocytogenes grown on medium containing a selective mixture. On this medium Listeria monocytogoenes forms characteristics blue (pale blue, grey blue to dark blue) colonies without a yellow halo. RAPID‘L. mono detects Listeria monocytogenes in 24 hours and other Listeria species in 24 and 48 hours. The method is applicable for the enumeration of Listeria monocytogenes and for the detection of other Listeria species in a broad range of food and environmental samples. See the certificate here.
NordVal International Certificate 030 issued to Qualicon for " BAX® System PCR Assay for Salmonella" has been renewed. An extension study has been performed to ensure compliance with ISO 16140-2 and NordVal Validation Protocol 1. The Bax® System for detection of Salmonella is targeting a specific bacterial DNA fragment, which is specific for Salmonella and which is not present in any other bacteria, and hence is an indicator of the presence of Salmonella. The method is applicable for the detection of Salmonella spp. in a broad range of foods, animal feed and environmental samples. See the certificate here.
NordVal International Certificate 041 "Salmonella detection method by real-time PCR" from Eurofins has been renewed.
The method describes a shortened pre-enrichment in buffered peptone water followed by DNA extraction and subsequent real-time PCR analysis. The Salmonella detection method using DNA extraction by boiling or King Fisher is applicable for raw meat and swabs from cattle and pig carcasses. The Salmonella detection method using DNA extraction by King Fisher is also applicable for poultry faeces sock swabs. See the certificate here.
New NordVal Certificate 048 issued for LactoSens® 0.01%. DirectSens® has developed a biosensor for the determination of residual lactose in lactose-free milk products. Using a specific enzyme and an electrochemical measurement, the lactose concentration is detected using the LactoSens® Reader (LR01) with sensors from the LactoSens® Test Kit (LK0225). Further information is given in the certificate. A validation of LactoSens has been performed according to the NordVal Validation Protocol 2. The performance of the LactoSens® 0.01% assay was compared against the reference method ISO 22662 IDF 198 Milk and milk products – Determination of lactose content by HPLC. The results obtained are satisfactory and document that there is no statistical difference in the performances between the methods for lactose in milk (and milk products) for levels at or above 0.01%. LactoSens is validated for cow/bovine milk. See the certificate here.